Year: 2025
Type: article
Abstract: The aim of this study was to investigate mutations in the rpoB and embB genes using molecular methods in Mycobacterium tuberculosis isolates phenotypically resistant to rifampicin and/or ethambutol. Drug susceptibility testing of 110 isolates collected in Turkey between 2005 and 2014 was performed using the modified agar proportion method. Molecular analysis was conducted on 28 selected isolates, including 7 rifampicin-resistant strains. Mutations were detected within the Rifampicin Resistance-Determining Region (RRDR) between codons 507–533 of the rpoB gene. The most common mutation was Ser531Leu (TCG→TTG), found in 10 strains; one strain had a His526Asn (CAC→AAC) mutation. These mutations were considered important molecular markers of rifampicin resistance. Rapid molecular detection of these mutations is important for early and effective tuberculosis treatment planning.
Institution: Bursa Uludağ Üni̇versi̇tesi̇
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4413910106