Feature-driven whole-tissue imaging with subcellular resolution
Year: 2025
Type: article
Abstract: Existing microscopy approaches are often unable to identify and contextualize rare but biologically meaningful events due to limitations associated with simultaneously achieving both high-resolution imaging and a cm-scale field of view. Here, we present multiscale cleared tissue axially swept light-sheet microscopy (MCT-ASLM), a platform combining cm-scale imaging with targeted high-resolution interrogation of intact tissues in human-guided or autonomous modes. Capable of capturing fields of view up to 21 mm at micron-scale resolution, MCT-ASLM can seamlessly transition to a targeted imaging mode with an isotropic resolution that approaches ∼300 nm. This versatility enables detailed studies of hierarchical organization and spatially complex processes, including mapping neuronal circuits in rat brains, visualizing glomerular innervation in mouse kidneys, and examining metastatic tumor microenvironments. By bridging subcellular- to tissue-level scales, MCT-ASLM offers a powerful method for unraveling how local events contribute to global biological phenomena.
Source: Cell Reports Methods
Authors: Jinlong Lin, Zach Marin, Xiaoding Wang, Hazel M. Borges, Qionghua Shen, Pierre-Emmanuel Y. N’Guetta, Xuemei Luo, Baylee A. Porter, Yuanyuan Xue, Md Torikul Islam, Tai Ngo, Doreen Idonije, Seweryn Gałecki, Arin B. Aurora, Hu Zhao, Suzanne D. Conzen, Sean J. Morrison, Shuang Liang, Zhenyu Zhong, Lori L. O’Brien, Kevin M. Dean
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $4,824
Funders: Howard Hughes Medical Institute, American Heart Association, Cancer Prevention and Research Institute of Texas, Lyda Hill Philanthropies, National Cancer Institute, National Institute of General Medical Sciences, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Neurological Disorders and Stroke
Awards: G251551830, Mechanisms of melanoma metastasis, G4567063627, Investigating the mechanism of NLRP3 inflammasome hyperactivation during hepatocellular carcinoma development, Estrogen and glucocorticoid receptor crosstalk in ER+ breast, G602583128, G6218943888, G6509056033, G6557920667, Recruitment of First-Time, Tenure-Track Faculty Members, Uncovering Roles Of Renal Nerves During Kidney Development, Restoring TREM2-Depedent Efferocytosis to Inhibit Obesity-Induced Liver Inflammation and Cancer Development, Trophic interactions directing proper kidney development, Improving Focused Ultrasound Mediated Viral Gene Therapy Delivery, UT Southwestern Medical Center Simmons Comprehensive Cancer Center, Mechanisms and Consequences of PAX2 Inactivation in the Initiation of Endometrial Carcinogenesis
OpenAlex ID: W4413911950