Work

In vitro assessment of copper nanoparticles gel efficacy as a futuristic drug delivery agent against periodontal pathogens

Year: 2025

Type: article

Abstract: Copper nanoparticles (Cu-NP) have garnered attention in dentistry due to their potential antimicrobial properties. The study aimed to assess the minimum inhibitory and bactericidal concentration, cytotoxicity against periodontal and mouse fibroblasts by MTT assay, time-kill assay against periodontal pathogens, antibiofilm formation by the cell culture method, and anti-inflammatory and antioxidant activity of the prepared Cu-NP gel. Minimum inhibitory concentration (MIC) value ranged from 0.4–3.12 μg/mL, whereas MBC value ranged from 0.4–25 μg/mL for periodontal pathogens. IC50 value of Cu-NP against mouse fibroblasts (L939) was 49.12±1.67, and against periodontal fibroblasts at 3.12%, displaying 98% of cell viability. Time-kill assay showed no growth of Prevotella intermedia and Tannerella forsythia after 10 min, Aggregatibacter actinomycetemcomitans at 30 min, Fusobacterium nucleatum at 4 h, and Porphyromonas gingivalis at 24 h. Antioxidant assay determined inhibition at 55.6%, showing weak anti-inflammatory and anti-biofilm activity. Cu-NP gel is cost-effective, eco-friendly, and holds promise for combating infections and promoting tissue regeneration.

Source: Journal of Taibah University for Science

DOI: 10.1080/16583655.2025.2549161

Authors: Swapna Arunkumar Mahale, Prasad Dhadse, Arunkumar Mahale, Shahabe Saquib Abullais

Institutions: Datta Meghe Institute of Higher Education and Research, King Khalid University

Language: English

Topic: Nanoparticles: synthesis and applications

Subfield: Materials Chemistry

Field: Materials Science

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $944