Human umbilical cord mesenchymal stem cell overexpressing granulocyte-colony stimulating factor alleviates endometrial injury in rats
Year: 2025
Type: article
Abstract: Background: The utilization of human umbilical cord-derived mesenchymal stem cells (UC-MSCs) or granulocyte-colony stimulating factor (G-CSF) as standalone therapies for the treatment of endometrial injury has been extensively documented.However, the possibility of using UC-MSCs overexpressing G-CSF for the treatment of endometrial injury remains underreported.Methods: UC-MSCs were isolated from human umbilical cords and characterized by flow cytometry.Subsequently, UC-MSCs overexpressing G-CSF were established.The rats with endometrial injury were intravenously transplanted with UC-MSCs or UC-MSCs overexpressing G-CSF.The uterine histology was examined through Masson's trichrome staining and hematoxylin & eosin staining assays.Immunohistochemical assessment of pan-keratin (AE1/AE3) and vascular endothelial growth factor (VEGF) levels was conducted in uterine tissues.Western blotting was employed to ascertain the levels of fibrosis-, angiogenesis-, inflammation-, apoptosis-, and endometrial receptivity-related proteins.Results: UC-MSCs overexpressing G-CSF effectively mitigated the pathological characteristics of damaged endometrium, enhanced angiogenesis, and promoted epithelial growth.Additionally, treatment with UC-MSCs overexpressing G-CSF significantly modulated the levels of proteins associated with fibrosis, angiogenesis, inflammation, apoptosis, and endometrial receptivity.This highlights the therapeutic potential of UC-MSCs in regulating key processes involved in angiogenesis, fibrosis, inflammation, apoptosis of endometrial stromal cells, and enhancing endometrial receptivity during the progression of endometrial injury.Conclusion: Transplantation of UC-MSCs overexpressing G-CSF contributed to the repair of endometrial injury, probably through regulating multiple signaling pathways such as fibrosis, angiogenesis, inflammation, apoptosis, and endometrial receptivity.To some extent, these findings provide a theoretical foundation for the use of endometrial injury therapy in clinical settings.
Authors: Ying Lyu, Liyan Ye, Ying Wang, Xiujuan Zheng
Language: English
Subfield: Genetics
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4413920324