Work

Cryptosporidium exports a mucin glycoprotein into the microvilli of intestinal epithelium

Year: 2025

Type: article

Abstract: Cryptosporidium causes severe diarrhea in humans and animals. Mucin-like glycoproteins play a critical role in parasite attachment and invasion and therefore serve as potential protective antigens against reinfection. Muc25 is a highly polymorphic mucin that has been associated with differences in host infectivity in comparative genomic analyses. To study the function of Muc25, we determined its localization and secretion in Cryptosporidium parvum by genome editing. Endogenous gene tagging revealed that Muc25 is stored in small granules of sporozoites and secreted into host microvilli after invasion. Deletion of the signal peptide affected Muc25 localization and secretion, as the Muc25ΔSP protein was localized to the membrane in sporozoites and remained within intracellular life stages. In addition, a Muc25 knockout strain (ΔMuc25) was easily generated, indicating that Muc25 is not critical for parasite survival. However, the ΔMuc25 strain showed reduced growth in HCT-8 cells, and the survival time was prolonged in GKO mice infected with ΔMuc25 compared to those infected with Muc25–3 HA. Transcriptome analysis revealed that ΔMuc25 parasites caused less damage to host cells in vitro than Muc25–3 HA parasites. Taken together, these data provide evidence for the export of a Cryptosporidium protein to host microvilli and demonstrate that such manipulation of the host cell response may be involved in parasite pathogenesis.

Source: Virulence

DOI: 10.1080/21505594.2025.2553780