Work

Report of bovine leukemia virus (BLV) in situ detection in primary cultures of bovine mammary epithelial cells

Year: 2025

Type: article

Abstract: A BLV infected cow, with high proviral load and persistent lymphocytosis, was used for this study. After euthanasia, nipple epithelial material was collected, disaggregated, and primary cultures were established. After 5 passages, viral proteins could be detected by immunocytochemistry, indicating that the virus is capable of expressing proteins in the cytoplasm of the host cell. Proviral load was quantified by qPCR in blood and milk of the infected animal. The presence of viral particles in milk and meat for human consumption has been described previously Therefore this report warns us of the possibility of BLV transmission to humans, with the potential risk of zoonotic disease.

Source: Analecta Veterinaria

DOI: 10.24215/15142590e100

Authors: Anahí de los Ángeles Chamorro, Pedro Edgardo Morán, María Victoria Nieto Farías, Pamela Anahí Lendez, Guillermina Laura Dolcini, María Carolina Ceriani

Institutions: Universidad Nacional del Centro de la Provincia de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas

Language: English

Topic: T-cell and Retrovirus Studies

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: diamond

Funders: Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional del Centro de la Provincia de Buenos Aires, Sociedad Española de Catálisis