Year: 2025
Type: article
Abstract: Intercellular pathways of viral infection in host cells offer advantages, such as efficiency of viral spread and immune surveillance evasion, compared to cell-free viral infection. Therefore, some enveloped viruses present both cell-to-cell and cell-free forms of infection in the host organisms. In this study, we investigated the occurrence of Ebola virus (EBOV) and Marburg virus (MARV) nucleocapsid exchange in vitro between interconnected Huh7 cells using live-cell imaging methods. Moreover, through plasmid transfection methods, we demonstrated that nucleocapsid-like structures (NCLSs) formed with EBOV NP, VP35, VP24, and VP30 proteins can also be transported intercellularly to non-transfected cells through cell-to-cell contact regions in a process involving interaction with the host cell actin cytoskeleton. Our results provide further evidence of cell-to-cell transport as a mechanism of filovirus spread and support the need for further research in this field to develop new intervention methods targeting this transmission pathway.
DOI: 10.3390/ijms26178485
Institution: Nagasaki University
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,499
Awards: G1054974808, G1185511082, G1271143712, G1459112527, 細胞生物学的アプローチで高病原性ウイルスの細胞内動態を可視化する研究開発, G4020521918, G4800164055, G5789080598, G643045179, G7034555318, G8153011623, G826749543, Reveling the intra-cellular replication machinery of Severe fever with thrombocytopenia syndrome virus by using live-cell imaging., G8407146073, フィロウイルス粒子形成機構の解明, G871049282, G8849845433, G976947312
OpenAlex ID: W4413927186