Work

Paeoniflorin inhibits degranulation of mast cells through suppressing the cGAS/STING signaling pathway

Year: 2025

Type: article

Abstract: Introduction: Allergic rhinitis is an allergic disease that affects the nasal mucosa, and its incidence has been increasing significantly in recent years. Paeoniflorin (PF) is the main chemical component of total glucosides of paeony and is reported to have an excellent effect on anti-allergic inflammation. In this study, we aimed to investigate the mechanism of PF regulating the degranulation of mast cells induced by dinitrophenol (DNP)-human serum albumin (DNP-HSA). Material and methods: -hexosaminidase, and intracellular calcium level, and the signaling pathway was screened through western blot assay and molecular docking. Results: -hexosaminidase, and reduced the intracellular calcium level in mast cells with DNP-HSA treatment. In addition, PF downregulated the protein levels of cGAS and STING to inactivate the cGAS/STING signaling pathway, in which the inhibitory effects of PF on mast cells were reversed by 5,6-imethylxanthenone-4-acetic acid (DMXAA, a STING agonist). Conclusions: In conclusion, PF inhibits degranulation of mast cells through the cGAS/STING signaling pathway, which provides a theoretical basis for the application of PF in allergic rhinitis therapy.

Source: Central European Journal of Immunology

DOI: 10.5114/ceji.2025.153658

Authors: Guanli Song, Guanbo Song, Xiaji Zhou, Lei Zhang

Institutions: Jining Traditional Chinese Medicine Hospital, China Academy of Chinese Medical Sciences, Guang’anmen Hospital

Language: English

Topic: Mast cells and histamine

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $161