Work

An LC-MS/MS Method for the Quantification of Tobacco-Specific Carcinogen Protein Adducts

Year: 2025

Type: article

Abstract: 4-(Methyl-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and its major metabolite 4-(methylnitrosamino)-l-(3-pyri-dine)-l-butanol (NNAL) are tobacco-specific lung carcinogens. Methods have been developed to quantify NNK- and NNAL-specific DNA adducts in pre-clinical samples but are less feasible to translation due to limited access to target tissues for sufficient DNA. NNAL-specific DNA or protein adducts have never been detected in clinical samples, which are critical to support the physiological relevance of NNAL bioactivation and carcinogenesis. We reported a sensitive and specific LC-MS/MS method to quantify hydrolyzed NNAL adduct, 1-(3-pyridinyl)-1,4-butanediol (PBD). The method was applicable to variety of biological samples, to assess tobacco exposure and estimate NNAL bioactivation.

Source: Chemical Research in Toxicology

DOI: 10.1021/acs.chemrestox.5c00149

Authors: Breanne Freeman, Chengguo Xing

Institution: University of Florida

Language: English

Topic: Carcinogens and Genotoxicity Assessment

Subfield: Cancer Research

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: Florida Department of Health, Division of Cancer Prevention, National Cancer Institute, National Cancer Institute, Cancer Center, University of Florida Health