Work

Overexpression of potassium channel Kcna5 alters skeletal patterning in the zebrafish regenerating fin

Year: 2025

Type: article

Abstract: Skeletal patterning relies on a complex network of molecular and genetic regulators. However, our understanding of pathways governing joint placement and morphogenesis remains incomplete. Prior studies have demonstrated that medially located Cx43 mediated gap junctional intercellular communication (GJIC) inhibits joint formation in the adjacent lateral skeletal precursor cells, and thereby regulates skeletal patterning. Here, we test the model that Cx43-GJIC regulates joint formation by propagating changes in membrane potential (ΔVm). To determine if ΔVm is sufficient to influence joint formation, we generated a transgenic line that expresses the X. laevis voltage-gated channel, shaker-related subfamily, member 5 (kcna5) behind the temperature-inducible heat shock protein 70 (hsp70) promoter. Using this line, we demonstrate that kcna5 overexpression delays evx1 expression and causes longer segments. Moreover, the increased segment length in response to Kcna5 overexpression requires Cx43. These findings strongly support the model that the propagation of ΔVm via Cx43 gap junctions contributes to joint formation, and therefore skeletal patterning, in the zebrafish regenerating fin.

Source: Developmental Biology

DOI: 10.1016/j.ydbio.2025.09.001

Authors: Alexander W. Seaver, Xinxhao Li, M. Kathryn Iovine

Language: English

Topic: Developmental Biology and Gene Regulation

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,550

Funders: Lehigh University, National Institutes of Health, National Institute of Child Health and Human Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development

Awards: G1389757541, Understanding how Connexin43 regulates joint formation in the regenerating zebrafish fin, G3045827408