Liquid-phase epitaxial grown UiO-66 as a bifunctional platform for electrochemical Aptasensing of cardiac troponin I
Year: 2025
Type: article
Abstract: The construction of multifunctional nanointerface bearing rich active site for aptamer immobilization and effective signal transformation is critical for the development of high-performance electrochemical aptasensor. Herein, a layer of p -mercaptobenzoic acid ( p -MBA) was self-assembled on the surface of bare gold electrode (AuE), acting as a scalfod for the consequent liquid-phase epitaxial growth of Universitetet i Oslo-66 (UiO-66) metal-organic framework via immersion in zirconium ions (Zr 4+ ) and p -terephthalic acid (PTA) ligand. Then, the UiO-66 was utilized as a bifunctional platform for immobilization of aptamer probe for cardiac troponin I (cTnI) and the electrochemical signal transformation as an electrocatalyst. Electrochemical experiments showed that UiO-66 has catalase-like properties for electrocatalytic reduction of H 2 O 2 , while when the aptamer interacts with cTnI to form a complex, the catalytic acttivity is inhibited. The catalytic currents in chronoamperometric measurements showed a good linear relationship with the negative logarithm of the target concentration in the range of 100 fg mL −1 –100 ng mL −1 , and the detection limit was 13 fg mL −1 . The aptasensor has good selectivity for cTnI and can be used for the detection of cTnI in human serum samples. • UiO-66 was prepared on electrode surface by liquid-phase epitaxial growth strategy. • UiO-66 was used for aptamer immobilization and signal transformation. • UiO-66 exhibits catalase-like activity to catalyze H 2 O 2 reduction for cTnI detection. • The aptasensor shows a low detection limit of 13 fg mL −1 . • The method is reliable for the detection of cTnI in actual human serum samples.
Source: Bioelectrochemistry
Authors: Yuan Zhu, Sifan Wang, Haizhu Yang, Feng Gao, Qingxiang Wang
Institution: Minnan Normal University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,970
OpenAlex ID: W4413970068