Work

An EJC-independent novel isoform of MAGOH: the MAGOH-Δ37 isoform and its interactome capture

Year: 2025

Type: article

Abstract: The Exon-Junction Complex (EJC) is essential for post-transcriptional gene regulation, with MAGOH as one of its core components, known for its involvement in mRNA surveillance and translation. In this study, we characterize an evolutionarily conserved, alternatively spliced isoform of MAGOH, i.e. MAGOH-Δ37, which has remained largely unexplored until now. Our study reveals that, unlike canonical MAGOH, MAGOH-Δ37 does not interact with known EJC proteins such as EIF4A3, RBM8A, RNPS1, or SAP18, suggesting a distinct functional role independent of the EJC. Intriguingly, both MAGOH and MAGOH-Δ37 associate with proteins implicated in neurodegenerative disorders, suggesting EJC-independent interactions. Furthermore, MAGOH and its isoform MAGOH-Δ37 interact with ubiquitin and were found to be upregulated upon proteasomal inhibition. The association of the isoforms with a distinct polyubiquitin signature suggests a potential role in the ubiquitin-proteasome system, either as targets or binding partners. These findings provide new insights into the roles of MAGOH isoforms in stress-related pathways, with implications for their involvement in neurodegenerative conditions.

Source: Biochemical and Biophysical Research Communications

DOI: 10.1016/j.bbrc.2025.152540

Authors: Ayushi Rehman, Raja Tamilselvan, Henrique Baeta, Gourab Chatterjee, Kusum Kumari Singh

Institutions: Indian Institute of Technology Guwahati, University of Freiburg

Language: English

Topic: RNA Research and Splicing

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,110

Funders: Department of Biotechnology, Ministry of Science and Technology, India, Ministry of Education, India, Science and Engineering Research Board