Year: 2025
Type: preprint
Abstract: This protocol is for fixation of Aiptasia (Exaiptasia diaphana) larvae and subsequent staining of microtubules, filamentous actin and nuclei. The resulting samples can be imaged under a widefield fluorescence microscope with sufficient image quality to assess basic morphological features such as size, gastric cavity and pharynx, cilia and apical tuft. For highest quality images, a confocal laser-scanning microscope can be used.
Author: Christian CR Renicke
Institution: Stanford University
Language: English
Subfield: Paleontology
Field: Earth and Planetary Sciences
Domain: Physical Sciences
Open Access status: gold
OpenAlex ID: W4413983539