Year: 2025
Type: article
Abstract: We introduce a Fluorescence Integrated Single-Cell Analysis Script (FISCAS) that combines fluorescence microscopy with MALDI-MSI to streamline single-cell analysis. FISCAS enables automated selection of tight measurement regions, thereby reducing the acquisition of off-target pixels, and makes use of established algorithms for cell segmentation and coregistration to rapidly compile single-cell mass spectra.
Source: BIOspektrum
Authors: Jan Schwenzfeier, Jens Soltwisch
Institution: University of Münster
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $3,190
OpenAlex ID: W4413991071