Work

Spatial and functional characterization of IL1RL1+ mast cells reveals immune regulatory roles in renal cancer

Year: 2025

Type: article

Abstract: BACKGROUND: Interleukin-1 receptor-like 1 (IL1RL1, also known as ST2) plays a critical role in immune regulation. Pan-cancer analysis has revealed that IL1RL1 is closely associated with cellular immune functions; however, its role in clear cell renal cell carcinoma (ccRCC) and the tumor microenvironment (TME) remains poorly defined. METHODS: We analyzed IL1RL1 expression patterns using data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) were employed to investigate the cellular localization and biological functions of IL1RL1 in ccRCC. In addition, IL1RL1 knockout experiments were conducted to explore its potential role in antigen presentation. RESULTS: mast cells and T cells within the TME. Furthermore, IL1RL1 knockout led to a reduction in HLA-A expression, providing functional evidence for its involvement in antigen presentation. CONCLUSION: T cell activation, offering new insights into its potential as a prognostic biomarker and therapeutic target in renal cancer.

Source: Molecular and Cellular Probes

DOI: 10.1016/j.mcp.2025.102049