Micronutrients content directly impact cerulenin production by Sarocladium oryzae BRM 59907
Year: 2025
Type: article
Abstract: Fungal secondary metabolites, renewable compounds with diverse bioactivities, offer potential for medicines, colorants, and agrochemicals. Their biotechnological production bottlenecks, such as low production and variable yield, can be overcome through bioprocess engineering techniques and experimental designs. Cerulenin, a broad-spectrum antimicrobial compound produced by Sarocladium oryzae , can be obtained with higher yields and lower costs using these strategies. In this work, cerulenin production by Sarocladium oryzae BRM 59907 was optimized considering its micronutrient requirements. Plackett-Burman and Doehlert designs showed that magnesium supplementation inhibits cerulenin biosynthesis, while the addition of iron source stimulates it. Thus, it was possible to reach an optimal condition (20 g L −1 glycerol, 5 g L −1 glucose, 2.5 g L −1 peptone, 0.025 g L −1 NaCl, 0.029 g L −1 FeCl 3 , 28 ºC and 150 rpm) with a reduction of 74 % in the cerulenin production cost considering only culture medium components from the original condition. Bioprocess scale-up in a bioreactor resulted in a cerulenin productivity of 1.39 mg L −1 h −1 in 72 h. This work presents the first bioprocess that efficiently utilizes glycerol as the primary carbon source for the secondary metabolite production. Magnesium supplementation increased cell growth but inhibited cerulenin production, while iron addition increased the concentration of cerulenin.
Source: Process Biochemistry
Authors: João Rogério Borges de Amorim Rodrigues, Marcio Vinicius de Carvalho Barros Cortes, Elisa D.’Avila Costa Cavalcanti, Denise Maria Guimarães Freire
Institution: Universidade Federal do Rio de Janeiro
Language: English
Subfield: Pharmacology
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $3,970
Funders: National Institute of Technology, Raipur, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Conselho Nacional de Desenvolvimento Científico e Tecnológico, Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro, Financiadora de Estudos e Projetos, Agência Nacional do Petróleo, Gás Natural e Biocombustíveis, Universidade Federal do Rio de Janeiro
Award: G2535661932
OpenAlex ID: W4414001992