Overexpression of Vitreoscilla hemoglobin gene enhances flooding resistance in Brassica napus
Year: 2025
Type: article
Abstract: Flooding can lead to oxygen deprivation in rapeseed, negatively affecting its growth and development and ultimately reducing yields. Vitreoscilla hemoglobin (VHb), a bacterial hemoglobin with a high oxygen-binding affinity, plays a key role in enhancing oxygen uptake and metabolic efficiency under low-oxygen conditions. Through genetic transformation, we overexpressed the VHb gene in rapeseed, which resulted in significant improvements in survival rate, root length, and biomass under submerged conditions. Additionally, we observed that transgenic plants developed adventitious roots in response to submergence stress. These transgenic plants also exhibited increased activities of ethanol dehydrogenase and pyruvate decarboxylase—enzymes associated with anaerobic respiration. Our findings indicate that VHb enhances flooding tolerance in rapeseed by promoting adventitious root formation and strengthening the plant's capacity for fermentation metabolism under anaerobic conditions.
Source: Oil Crop Science
Authors: Jiaxin Liu, Jinfeng Wu, Xinhong Liu, Lili Liu, Mingli Yan, Bao Li
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
Funders: Key Project of Research and Development Plan of Hunan Province, Hunan Provincial Science and Technology Department, Science and Technology Program of Hunan Province, Key Research and Development Program of Hunan Province of China, Hunan Academy of Agricultural Sciences, Key Technologies Research and Development Program, National Key Research and Development Program of China, Science and Technology Support Plan for Youth Innovation of Colleges and Universities of Shandong Province of China
OpenAlex ID: W4414010610