Work

Comparison of 4 different RNA sources from lactating dairy cows to assess the mammary transcript abundance

Year: 2025

Type: article

Abstract: ) were related to immune function and were mainly expressed in RNA from milk somatic cells, with fewer transcripts from milk fat and few to no transcripts from mammary tissue or blood leukocytes. Relative transcript abundance in RNA from milk fat was comparable to transcript abundance in RNA from mammary tissue for milk-related genes, but for immune-related genes, it was more similar to RNA from milk somatic cells than from mammary tissue or blood leukocytes. Thus, transcript abundance of the assessed genes was uniquely controlled between each of the 4 tissue sources. Using RNA from milk fat may be beneficial for studies investigating lactational and immunological responses of the mammary gland based on its similar transcript abundance patterns compared with RNA from both mammary tissue and milk somatic cells.

Source: JDS Communications

DOI: 10.3168/jdsc.2025-0768

Authors: E.M. Shangraw, M.C. Lucy, T.B. McFadden

Institution: University of Missouri

Language: English

Topic: Milk Quality and Mastitis in Dairy Cows

Subfield: Agronomy and Crop Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,310