Small-scale in situ Hi-C protocol for early embryos to resolve the three-dimensional genome structure
Year: 2025
Type: article
Abstract: High-throughput chromosome conformation capture (Hi-C) provides genome-wide insights into chromatin interactions within the three-dimensional structure of the nucleus, making it a powerful tool for studying genome architecture. Here, we provide a modified in situ Hi-C protocol for small cell numbers, utilizing 50–100 embryonic cells at the 8-cell stage to investigate chromatin organization during bovine early embryonic development. This protocol overcomes the challenges of limited sample availability and offers valuable insights into chromatin dynamics during bovine early embryogenesis. For complete details on the use and execution of this protocol, please refer to Zhang et al. 1
Source: STAR Protocols
Authors: Linjie Song, Jingcheng Zhang, Linqian Pan, Shengjie Sun, Binqiang Shi, Hexu Zhang, Hengkuan Li, Zixiao Liu, Linmi Li, Yong Zhang, Gang Ren
Institution: Northwest A&F University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,550
Awards: G2825007974, G7229844919
OpenAlex ID: W4414057136