BBX10 interacts with PIF1 to prevent photo-oxidation and to promote the greening process
Year: 2025
Type: article
Abstract: Seedlings emerged from the covering soil immediately undergo de-etiolation, ensuring plants switch from heterotrophic to photoautotrophic growth. This transition is essential for seedling development and survival. However, the underlying mechanism remains largely obscure. Here, we identified that B-box PROTEIN 10 (BBX10) inhibits the biosynthesis of protochlorophyllide in darkness and accumulation of reactive oxygen species (ROS) upon light exposure, hence leading to the increase of seedling greening and survival during de-etiolation. BBX10 interacts with PHYTOCHROME INTERACTING FACTOR 1 (PIF1) and enhances its DNA-binding affinity and transcriptional repression or activation activity toward target genes GENOMES UNCOUPLED 5 (GUN5) and ASCORBATE PEROXIDASE 2 (APX2), respectively. Genetically, BBX10 acts upstream of PIF1, GUN5, and APX2 in regulating of greening. Together, our study reveals that the BBX10-PIF1 transcriptional module inhibits the protochlorophyllide biosynthesis in darkness and ROS accumulation upon light irradiation, through which seedlings protect themselves against photo-oxidative damage upon emerging from the soil.
Source: Cell Reports
Institutions: Nanjing Agricultural University, Zhejiang University
Language: English
Topic: Light effects on plants
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $5,620
Funders: Natural Science Foundation for Distinguished Young Scholars of Anhui Province, National Natural Science Foundation of China, Chinese Academy of Sciences, Nanjing Agricultural University, Jiangsu Collaborative Innovation Center for Modern Crop Production, National Key Research and Development Program of China, Natural Science Foundation of Jiangsu Province for Distinguished Young Scholars
OpenAlex ID: W4414078204