Work

Adapting a 96-well liquid culture assay to a colonial growth confirmation assay utilizing a 3D printed guide-frame system for a 96-well replicator

Year: 2025

Type: article

Abstract: Some bacteria are not suited to assays requiring visual growth in broth; it may be useful to transfer cells from these assays to solid media to confirm growth. Here, we present a novel guide-frame system for accurate transfer of a 96-well assay to solid agar using a replicator.

Source: Journal of Microbiological Methods

DOI: 10.1016/j.mimet.2025.107246

Authors: Micah A. Lewis, Eric S. Adams, Elizabeth A. McMillan

Institutions: Agricultural Research Service, U.S. National Poultry Research Center, United States Department of Agriculture

Language: English

Topic: Bacterial Identification and Susceptibility Testing

Subfield: Clinical Biochemistry

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,120

Funder: U.S. Department of Agriculture