Temporal and Spatial Dynamics in the Regulation of Myocardial Metabolism During the Ischemia-Reperfusion Process
Year: 2025
Type: article
Abstract: Although cardiac metabolic adaptation has been observed in response to the ischemia-reperfusion, the specific temporal and spatial changes occurring in the main regulators of myocardial glucolipid metabolism in the infarcted heart have not been fully characterized. Myocardial infarction (MI) was induced in female swine by transient coronary occlusion. The study design consisted of one control and four MI groups (no reperfusion, 1 min, 1 week, and 1 month after reperfusion). Metabolites obtained from the coronary sinus were determined at baseline, during ischemia, and after coronary reperfusion. mRNA expression of genes related to beta-oxidation and glucose transport were quantified in the five experimental groups and in three myocardial regions (infarcted, adjacent, and remote). In the coronary sinus, reduced glucose and increased lactate levels were detected during ischemia and soon after reperfusion. However, non-esterified fatty acids increased during reperfusion. A general upregulation of genes implicated in glycolysis and beta-oxidation occurred during ischemia and few minutes after reperfusion. Contrarily, heightened mRNA expression of glucose transporters and decay in regulators of beta-oxidation were observed one week after coronary reperfusion. Glycolysis and beta-oxidation are activated during ischemia and few minutes after coronary reopening, while a shift from beta-oxidation to glycolysis is evidenced a few days afterwards.
DOI: 10.3390/ijms26188820
Institutions: Centro de Investigación Biomédica en Red, Karolinska University Hospital, Karolinska Institutet, Universitat de València, Hospital Universitari Germans Trias i Pujol, INCLIVA Health Research Institute, Hospital Clínico Universitario de Valencia, Andalusian Centre for Nanomedicine and Biotechnology, Hospital Clínico Universitario Virgen de la Victoria, Instituto de Investigación Biomédica de Málaga, Universidad de Málaga
Language: English
Subfield: Pathology and Forensic Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,499
Awards: G1070254935, G1077015544, G1148111505, G1350763705, G1948132165, G2807512858, G2979190613, G2988766369, G3520228682, G3818967781, G4518148856, G4605450927, G4768038251, G5268677451, G5768103789, Obstrucción microvascular tras un infarto de miocardio. Avances hacia una cuantificación automatizada, biomarcadores basados en técnicas ómicas y una estratificación de riesgo a largo plazo personalizada, G8072861482, G989731353
OpenAlex ID: W4414091658