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Clean up of Index PCRs using SPRI Beads v2

Year: 2025
Type: preprint
Abstract: This protocol describes the cleanup of index PCR products using SPRI bead-based size selection at a 1.4:1 bead-to-DNA ratio. The process includes bead binding, ethanol washes, DNA elution, and quantification using Qubit or other methods. The expected result is purified DNA libraries with concentration measurements for downstream analysis.
Language: English
Open Access status: gold
OpenAlex ID: W4414100969
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