Work

Protocol to perform assay for chromatin-bound condensates by exploratory sequencing in mouse embryonic stem cells

Year: 2025

Type: article

Abstract: Chromatin-related protein condensates are integral to nuclear functions including transcriptional regulation and chromatin structure. Here, we present a protocol for detecting the occupancy of chromatin-associated condensates using the assay for chromatin-bound condensates by exploratory sequencing (ACC-seq) in mouse embryonic stem cells (mESCs). The workflow encompasses cell preparation, condensate modulation, library construction, and comprehensive sequencing analysis, enabling unbiased, genome-wide mapping of potential condensate occupancy across diverse chromatin states. For complete details on the use and execution of this protocol, please refer to He et al . 1

Source: STAR Protocols

DOI: 10.1016/j.xpro.2025.104067

Authors: Zeran Jia, Jiawei Yu, Yinqing Li

Institutions: State Key Laboratory of Molecular Oncology, Tsinghua University

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,550