Work

Impact of Chronic Stress and Anxiety on Interleukin-6, Hypoxia-Inducible Factor-1α and on Pathological and Immunohistochemical Parameters in Breast Cancer

Year: 2025

Type: article

Abstract: OBJECTIVE: The Aim of this study is to evaluate stress and anxiety by rating scale in breast cancer (BC) patients and their association with interleukin-6 (IL-6), hypoxia-inducible factor-1α (HIF-1α), pathological and immunohistochemical parameters. METHODS: We analyzed serum concentrations of IL-6 and HIF-1α in 69 BC patients from western Algeria using ELISA technique, with optical density measured at a wavelength of 450 nm. Estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER2), and Ki-67 expression were assessed by immunohistochemistry. Venous blood samples were collected in heparinized tubes. Additionally, stress and anxiety levels were evaluated using standardized questionnaires and compared with the other measured parameters. RESULTS: Analysis of the correlations between the various variables revealed a positive correlation between anxiety and HIF-1α (p = 0.049, pearson correlation coefficient [PC] = 0.24), as well as between anxiety and the Scarff-Bloom-Richardson (SBR) grade (p = 0.037, PC = 0.26). A negative association was also observed between HIF-1α and HER2 status (p = 0.02, with the mean HIF-1α level in HER2-positive patients being 0.01 compared to 0.27 in HER2-negative patients). No significant association was found between IL-6 and stress (p = 0.4), nor between IL-6 and anxiety (p = 0.28). CONCLUSION: Our results show a positive correlation between anxiety and HIF-1α, which plays a crucial role in tumor angiogenesis, as well as with SBR grade. These findings suggest that anxiety may modulate tumor angiogenesis and contribute to increased tumor aggressiveness and grade. A negative association was also observed between HER2 and HIF-1α, which may be explained by a negative regulatory mechanism between the HER2 and HIF-1α signaling pathways. Furthermore, no association was found between stress or anxiety and IL-6 levels.

Source: Asian Pacific Journal of Cancer Prevention

DOI: 10.31557/apjcp.2025.26.9.3271