Work

Efficient Expression and Purification of Recombinant Mouse Dimeric IgA

Year: 2025

Type: article

Abstract: Immunoglobulin (Ig) A is the main antibody isotype found on mucosal surfaces in mammals, where it is predominantly present as a dimer. Here we provide an easy, scalable, efficient, and broadly applicable method to produce and purify monoclonal mouse dimeric IgA from single B cell Ig transcripts to study mucosal antibody responses at single-cell level.

Source: European Journal of Immunology

DOI: 10.1002/eji.70055

Authors: Antonia Geisse, Tao Zhang, Jonathan Schreiber, Kristina Markova, Sophie Burkhalter, Hedda Wardemann, Andrew J. Macpherson, Tim Rollenske

Institutions: University Hospital Bonn, University of Bern, University Hospital of Bern, German Cancer Research Center

Language: English

Topic: Monoclonal and Polyclonal Antibodies Research

Subfield: Radiology, Nuclear Medicine and Imaging

Field: Medicine

Domain: Health Sciences

Open Access status: hybrid

Estimated APC paid: $4,550

Funders: Deutsches Krebsforschungszentrum, University of Bern, Deutsche Forschungsgemeinschaft, UniBern Forschungsstiftung

Awards: G1285364889, SFB 1454: Metaflammation und Zelluläre Programmierung, G3620252990, G4549622993, IgAselect B Zell Selektion und Funktion in intestinalen Antikörperantworten gegen die Mikrobiota

OpenAlex ID: W4414168406

FWCI: 0.527

Cites: 16

Cited by: 1

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