Electric-stimulated melatonin-containing dressings for chronic wound treatment: impact on skin immunity and microbiota
Year: 2024
Type: dissertation
Abstract: This study explores the development of silk fibroin-based wound dressings containing melatonin, combined with iontophoresis, to enhance the healing of chronic wounds, especially in diabetes-associated conditions.The central hypothesis posits that the controlled release of melatonin from these electrically stimulated dressings can synergistically enhance complex tissue repair mechanisms while improving patient compliance.This control, achievable through iontophoresis, is expected to facilitate melatonin uptake by skin cells, leading to anti-inflammatory and antioxidant effects.Additionally, the dressings aim to sustain melatonin release, bolster the skin's immune system, and modulate the cutaneous microbiota.The study involved the preparation of homogeneous, transparent, and water vaporpermeable films composed of silk fibroin and hyaluronic acid, with or without melatonin.Detailed evaluations of the films' physicochemical, morphological, and mechanical properties were conducted.The application of iontophoresis significantly increased melatonin release, especially in the first 2 hours, and sustained this release for up to 96 hours.In vitro tests indicated that the films were non-cytotoxic to skin cells, including fibroblasts and keratinocytes.In vivo experiments demonstrated that iontophoresis application on chronic wounds improved healing, increasing the elasticity and resistance of healed skin, enhancing blood vessel density, and normalizing the inflammatory response.Histological analysis revealed a significant reduction in epidermal thickness and an increase in fibroblast density and type I collagen, indicating more efficient healing and the formation of higherquality tissue.Additionally, the treatments promoted a balanced inflammatory environment, which is essential for effective wound healing and the prevention of chronic infections.Combining melatonin-containing dressings with electrical stimulation via iontophoresis can enhance wound healing by providing a multifunctional approach.This study offers a promising perspective for developing advanced therapies for chronic wound treatment, with significant implications for clinical practice and improving patient quality of life.
Language: English
Topic: Wound Healing and Treatments
Subfield: Rehabilitation
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4414171539