Work

Microfluidic single‐cell technologies for immunotherapeutic target discovery: From design concepts to preclinical applications

Year: 2025

Type: article

Abstract: Immunotherapy, which modulates the patient's immune system to treat diseases, has revolutionized cancer therapy and is now being explored for autoimmune diseases. However, cellular heterogeneity in the immune system, tumor microenvironment, and inflamed tissues poses a major challenge to the efficacy of immunotherapies. This complexity underscores the need for a deeper understanding of disease mechanisms at single‐cell resolution. In recent years, microfluidic technologies have played a key role in enabling single‐cell analysis at transcriptomic and proteomic levels, leading to the discovery of new biomarkers for immunotherapy. In this review, we summarize the state‐of‐the‐art microfluidic platforms used for single‐cell omics, focusing on their conceptual principles and practical designs. We also briefly summarize the applications of microfluidic devices in the preclinical process of immunotherapeutic target discovery.

Source: Open Research (University of Surrey)

DOI: 10.1002/viw.20250065

Authors: Haopu Wang, Kangfu Chen, Zongjie Wang

Institutions: Beijing Institute of Technology, Northwestern University, Chan Zuckerberg Biohub Chicago

Language: English

Topic: Microfluidic and Bio-sensing Technologies

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,000