Work

Genetic Identification of Echinococcus granulosus in Slaughtered Domestic Animals from Two Northeastern Iranian Cities Using HRM and Sequencing Techniques

Year: 2025

Type: article

Abstract: Background: Hydatidosis is one of the common parasitic diseases common to both humans and animals with global spread. The disease is very important from both public health and economic perspectives. The parasite presents with highly diverse genetic features, including a different genotype which affect the life cycle of the parasite, its transmission routes, pathogenicity, immunogenicity, response to drugs, epidemiology and disease control. Therefore, we aimed to identify the genotype of hydatid cysts isolated from slaughtered animals in Sabzevar and Neyshabour slaughterhouses by sequencing and high-resolution melting curve analysis. Methods: Out of 35 hydatid cyst samples, including 19 from sheep (15 liver and 4 lung samples) and 16 from cattle (9 liver and 7 lung samples) were included in this study. After the extraction of protoscoleces and DNA, a high-resolution melting curve analysis method was used to identify the genotype of the samples using pre-determined G1, G3, and G6 standard samples. Results: gene considered a perfect biomarker in a molecular identification and phylogenetic study of this parasite. Conclusion: G1 was the most prevalent in samples of hydatid cysts (both sheep and cattle) in Sabzevar, while in Neyshabour samples, G1 and G6 were the most dominant types.

Source: Iranian Journal of Parasitology

DOI: 10.18502/ijpa.v20i3.19609

Authors: Hossein Elyasi, Amir Hossein Hajari, Ehsan Javaheri

Institution: Sabzevar University of Medical Sciences

Language: English

Topic: Parasitic infections in humans and animals

Subfield: Pathology and Forensic Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $250