Work

Rapid detection of Senecavirus A using a colloidal gold lateral flow strip

Year: 2025

Type: article

Abstract: Senecavirus A (SVA) could cause vesicular lesions and other symptoms in pigs, which has resulted in substantial economic losses to the swine industry. To establish a rapid detection method for SVA, an immunochromatographic lateral flow strip was constructed. First, the recombinant SVA VP2 protein was purified, and the monoclonal antibodies were prepared. 4B10 was coated onto the test line (T line), and 1E3 was colloidal gold-labeled to assemble the colloidal gold test strip. The constructed LFA strip showed a detection limit of 0.5 ng/mL, and no cross-reactivity with FMDV, PRRSV, CSFV and PCV2. Clinical samples validation showed 90 % concordance with RT-PCR results, indicated the reliability of this method. The developed lateral flow assay (LFA) provided a rapid and simple method for point-of-care testing of SVA. • A SVA colloidal gold lateral flow strip was developed. • The developed strip could sensitively detect SVA comparable to RT-PCR. • The detection by LFA can be completed in 15min.

Source: Biochemistry and Biophysics Reports

DOI: 10.1016/j.bbrep.2025.102240

Authors: Xiaomeng Pei, Jing Wang, Zhijing Wei, Yuanying Li, Meng Wu

Institutions: Hebei Academy of Sciences, Hebei Agricultural University

Language: English

Topic: Biosensors and Analytical Detection

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,700