Year: 2025
Type: preprint
Abstract: This protocol details the use of the Olympus (Evident Scientific) VS200 automated slide-scanning microscope for fluorescent imaging of fixed mouse, human, and nonhuman-primate tissues sections mounted on barcoded glass 1x3” or 2x3” slides. The instrument is capable of loading up to 210 1x3” glass slides, or 105 2x3” slides. The images are viewable in Evident’s proprietary OlyVIA image viewing software. This document is intended to describe routine use of the instrument and software, including the initial steps required to configure the software for image capture (section 8.3), which includes specific exposure times for the Viral Genetic Tools (VGT) primary screen.
Author: Allen Institute
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4414347834
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