Work

Proteomic Insights into the Interaction of Chitosan Nanoparticles with Canine MDCK Epithelial Cells

Year: 2025

Type: article

Abstract: Chitosan is considered an excellent biomaterial for epithelial healing treatment. However, information on its molecular interaction with cells at a molecular level is still lacking. Thus, in the present study, homemade synthesized chitosan nanoparticles (CS NPs) and their physicochemical characterization were examined; we found that NPs had average sizes of 15, 30, and 125 nanometers by modifying some variables in the synthesis protocols. It is worth noting that a crystalline structure was found on the smallest NPs, with an average size of 15 nm, as observed in high-resolution transmission electron micrographs. To study the in vitro interaction of CS NPs with Madin-Darby canine kidney (MDCK) cells, co-culturing was performed, and cell viability was assessed. We found that NPs were not toxic at concentrations of up to 400 µg/mL during the first 24 h. Additionally, quantitative mass spectrometry revealed the overexpression of several proteins induced by the co-culture of CS NPs with MDCK cells, and a proteomic analysis suggested two important things: possible clathrin-mediated endocytosis could be the interaction mechanism of CS NPS with MDCK, and proteins related to cytoskeleton formation and organization were overexpressed. Moreover, wound healing assays revealed that <125 nm> CS NPs yielded the best closure rates, where mitomycin was added to make sure that only cell migration occurred.

Source: Molecules

DOI: 10.3390/molecules30183815

Authors: Lorena Elizabeth Galván, Juan Carlos Osorio, Patricia Talamás, Salvador Gallardo

Institutions: Instituto Politécnico Nacional, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional

Language: English

Topic: Wound Healing and Treatments

Subfield: Rehabilitation

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,258