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Genomic DNA Extraction Protocol using DNeasy® UltraClean® Microbial Kit v1

Year: 2025

Type: preprint

Abstract: This protocol describes theextraction of high-quality genomic DNAfromPseudomans sp. usingmechanical and chemical lysis, followed bycolumn-based purification. The extracted DNA is suitable forPCR, sequencing, and other downstream applications. This protocol followsstrict contamination controlmeasures to ensure sample integrity and avoidDNA degradationduring extraction.

DOI: 10.17504/protocols.io.yxmvmyopnv3p/v1

Authors: Md Sahadat Ali, Fatima Tuz Zohora Mony, Jonathan D. Eisenback

Institution: Virginia Tech

Language: English

Topic: Genomics and Phylogenetic Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

OpenAlex ID: W4414351230

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