Work

Non-Invasive Three-Dimensional Cell Manipulation Technology Based on Acoustic Microfluidic Chips

Year: 2025

Type: article

Abstract: This study presents a non-invasive three-dimensional cell manipulation technique based on acoustic microfluidic chips, which generates acoustic flow fields through the vibration of micropillars induced by bulk acoustic waves to achieve precise multi-dimensional rotational manipulation of cells. Moreover, the characteristics of the acoustic flow field under linear, quasi-circular, elliptical, and higher-order vibration modes were intensively studied, and the rotational manipulation performance of polystyrene microbeads and cancer cells was optimized by adjusting the frequency and voltage. The results showed that the rotational speed and direction of the particles varied significantly in different vibration modes, with the particles and cells achieving the highest rotational speed in the elliptical vibration mode (frequency: 44.9 kHz, and voltage: 60 Vpp). In addition, the technique successfully achieved in-plane and out-of-plane rotation of cancer cells, and cell viability tests showed that 94% of the cells remained active after manipulation, demonstrating the low damage and biocompatibility of the method. This study provides a new, efficient, precise and gentle approach to three-dimensional manipulation of cells, which holds significant potential in biomedical research and clinical applications.

Source: Micromachines

DOI: 10.3390/mi16091068

Authors: Lin Lin, Yiming Zhen, Wang Li, Guoqiang Dong, Rongxing Zhu, Minhui Liang

Institutions: Guangxi University, Guangxi Key Lab of Manufacturing System and Advanced Manufacturing Technology

Language: English

Topic: Microfluidic and Bio-sensing Technologies

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,534