Glioma chemotherapeutic resistance is tied to membrane electrophysiological properties and glycosylation
Year: 2025
Type: article
Abstract: Diffuse gliomas are brain tumors that include oligodendroglioma, astrocytoma, and glioblastoma (GBM), the most common and deadly primary brain tumor. A major challenge in glioma treatment is resistance to the first-line chemotherapeutic, temozolomide (TMZ). Plasma membrane properties of cells with increased chemotherapeutic resistance are not well understood, despite the fact that the membrane is the first point of contact with the environment and greatly shapes cell behavior. Plasma membrane glycosylation impacts cell function, and we found significant differences in glycosylation of TMZ-resistant cells. We further identified plasma membrane electrophysiological properties predicting glioma cell TMZ resistance. We enriched cells with higher TMZ resistance by sorting glioma cells based on electrophysiological properties, indicating the relevance of membrane properties to chemotherapeutic resistance. These findings could lead to rapid separation methods for patient tumor cells, a better understanding of the molecular profiles of resistant cells, and novel treatment options for gliomas.
DOI: 10.1002/btm2.70069
Language: English
Subfield: Genetics
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,890
Awards: CIRM Scholars Comprehensive Research Training Program, Novel recognition and targeting of temozolomide resistant cells in glioblastoma, Targeting of Mitochondrial Lon Protease as a Novel Therapy for Glioblastoma, Cell surface glycosylation regulation of stem cell fate decisions, G9000077941, G9000094784, Human neural stem cell and endothelial cell reciprocal interactions govern cell function, Cancer Center Support Grant, A Training Program for Interdisciplinary Cancer Research, Training Program in Stem Cell Translational Medicine for Neurological Disorders
OpenAlex ID: W4414414556