Work

Label-free single-molecule optical detection

Year: 2025

Type: article

Abstract: Label-free optical detection techniques have established themselves as irreplaceable analytical tools across research and industry. The technological leap achieved by several detection modalities, reaching single-molecule sensitivity, opens new frontiers but also raises challenges in defining future biotechnological applications. This review discusses the fundamental principles of optical single-molecule detection, its methodological limitations, and the opportunities arising from recent technological advancements.

Source: npj Biosensing

DOI: 10.1038/s44328-025-00048-9

Authors: Tereza Roesel, Andreas Dahlin, Marek Piliarik, Laurence W. Fitzpatrick, Barbora Špačková

Institutions: FZU - Institute of Physics of the Czech Academy of Sciences, Chalmers University of Technology, Czech Academy of Sciences, Czech Academy of Sciences, Institute of Photonics and Electronics

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $2,590

Funders: Ministerstvo Školství, Mládeže a Tělovýchovy, Grantová Agentura České Republiky, Bundesministerium für Bildung und Forschung, Max-Planck-Gesellschaft