MdXTH30, an apple gene encoding endotransferase/hydrolase for xyloglucan, enhances plant resistance to drought, salt and pathogenic stresses
Year: 2025
Type: article
Abstract: Xyloglucan is the main component of hemicellulose in the cell walls of higher plants, which provides mechanical support. The XTH gene family encodes xyloglucan endotransferase/hydrolase, which is a key enzyme in cell wall remodeling. However, studies on XTH family-related genes in apples are rare. In this study, the MdXTH30 gene, isolated from apple ( Malus × domestica ), was responsive to abscisic acid, NaCl, and PEG 6000, and was localized to the cytoplasm according to a subcellular mapping technique. To further investigate the role of MdXTH30 in the stress response, we generated transgenic MdXTH30 apple calli and heterologously expressed this gene in Arabidopsis by Agrobacterium -mediated transformation. The results demonstrated that MdXTH30 enhanced resistance to drought, salt stress, and pathogens by regulating the expression of relevant genes in apple calli and Arabidopsis . These findings provide valuable insight into potentially important candidate genes for improving biotic and abiotic stress resistance at the cell wall level.
Institution: Shandong Agricultural University
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $1,000
OpenAlex ID: W4414417847