Work

Protocol for monitoring the growth of Solanum betaceum non-embryogenic callus using electrochemical impedance spectroscopy

Year: 2025

Type: article

Abstract: Callus cultures have important biotechnological applications. Here, we describe the use of electrochemical impedance spectroscopy (EIS) as a real-time and non-destructive approach to monitor callus growth. We describe the steps to obtain in vitro tamarillo clones from seeds and from these non-embryogenic calluses . We detail the steps for the preparation of the EIS apparatus and experiment and a parallel growth assay. We also provide complementary microscopy assays to complement the EIS experiment and outline the main data treatment steps. For complete details on the use and execution of this protocol, please refer to Caeiro et al. 1 • Instructions for induction of non-embryogenic callus • Protocol for callus growth assessment using electrochemical impedance spectroscopy • Description of complementary microscopy assays • Guidance on EIS for efficient and non-destructive monitoring of callus Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Callus cultures have important biotechnological applications. Here, we describe the use of electrochemical impedance spectroscopy (EIS) as a real-time and non-destructive approach to monitor callus growth. We describe the steps to obtain in vitro tamarillo clones from seeds and from these non-embryogenic calluses . We detail the steps for the preparation of the EIS apparatus and experiment and a parallel growth assay. We also provide complementary microscopy assays to complement the EIS experiment and outline the main data treatment steps.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2025.104100

Authors: André Caeiro, Jorge Canhoto, Paulo R.F. Rocha

Institutions: University of Coimbra, Laboratório Associado TERRA

Language: English

Topic: Plant tissue culture and regeneration

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,550