Work

The effect of poly I:C and LPS on in vivo and in vitro rainbow trout gill mucin production

Year: 2025

Type: article

Abstract: Mucins are key components of the mucosal barrier and contribute to innate defense by limiting pathogen access to epithelial surfaces. We investigated how exposure to lipopolysaccharide (LPS) and polyinosinic:polycytidylic acid (Poly I:C) affects mucin production in the gills of rainbow trout (Oncorhynchus mykiss) using metabolic labeling with N-azidoacetylgalactosamine. After intestinal administration, Poly I:C led to increased gill mucin production and upregulation of Interferon γ expression in the spleen, while LPS had no effect these aspects. In vitro, both LPS and Poly I:C increased mucin production in a differentiated gill epithelial cell line (RTgill-W1). Differences between in vivo and in vitro responses likely reflect local versus systemic exposure and immune involvement. This is the first study to use metabolic labeling to evaluate gill mucin turnover in teleosts, providing a more functional measure of mucin production than transcript or goblet cell quantification. These findings enhance our understanding of epithelial responses to immune stimuli in teleost mucosal surfaces.

Source: Fish & Shellfish Immunology

DOI: 10.1016/j.fsi.2025.110897

Authors: Sinan Sharba, Henrik Sundh, Kristina Sundell, Sara K. Lindén

Institution: University of Gothenburg

Language: English

Topic: Aquaculture disease management and microbiota

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $4,330