Mitochondria-Targeting Accumulation of 5MEF Probe for Early Diagnosis of Myocardial Infarction
Year: 2025
Type: article
Abstract: Myocardial infarction (MI) remains a leading cause of morbidity and mortality worldwide, necessitating the development of more precise diagnostic strategies beyond conventional biomarker detection and imaging techniques, which often suffer from limited specificity and sensitivity. Here, we report the design and evaluation of a mitochondria-targeting accumulation probe, 5MEF, for MI diagnosis. In vitro assays demonstrated that 5MEF exhibits high specificity and selective accumulation in H9c2 cells compared to that in HUVECs. In the rat MI model, 5MEF showed significant differences between the infarcted area and the noninfarcted area, indicating that 5MEF has diagnostic potential. Biosafety assessments conducted in vitro and in vivo revealed no significant cytotoxicity or adverse effects. In conclusion, our findings demonstrate that 5MEF is a highly promising molecular probe for the early detection of MI, providing a highly specific and safe diagnostic tool with a significant potential for clinical translation.
Source: Chemical & Biomedical Imaging
DOI: 10.1021/cbmi.5c00098
Authors: Qihang Ding, Ying Bai, Cheng Deng, Siwei Hua, Kun Qian, Xiang Wang, Huaner Ni, Jing Wang, Ping Gong, Zhen Cheng
Institutions: Union Hospital, Korea University, Union Hospital, Union Hospital, Huazhong University of Science and Technology, Tongji Hospital, Chinese Academy of Sciences, Shanghai Institute of Materia Medica, State Key Laboratory of Drug Research, Shanghai Jiao Tong University, Shanghai First People's Hospital, Shenzhen Institutes of Advanced Technology
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W4414426537