Year: 2025
Type: preprint
Abstract: To ensure consistent and high-quality maintenance of induced pluripotent stem cells (iPSCs) by implementing standardized protocols and adhering to good cell culture practices. This includes minimizing inter- and intra-batch variability, preventing microbial and cross-contamination, and optimizing culture conditions such as media composition, passaging frequency, and confluency thresholds to support reproducible and robust downstream applications including differentiation, gene editing, and transcriptomic profiling.
Authors: Jonghun Kim, In-Hyun Park, Hilary Blumberg
Institution: Yale University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4414434006
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