Work

Revealing plasma membrane protein dynamics in living plant cells with single-molecule tracking

Year: 2025

Type: article

Abstract: The behavior of proteins and other biomolecules in cellular environments is governed by complex molecular processes. Understanding their temporal dynamics and interactions with other biomolecules or cellular structures is essential for elucidating physiological functions. Single-particle tracking photoactivated localization microscopy (sptPALM) has emerged as a powerful single-molecule technique for investigating these processes with exceptional spatial and temporal resolution. In this Expert View, we introduce sptPALM and focus on its application in the plasma membrane of plant cells. Key aspects and advances in the technique, including instrumentation and data analysis, are discussed to equip researchers with the foundational knowledge required to establish and execute sptPALM experiments. Recent studies are highlighted to demonstrate the potential of sptPALM to advance our understanding of molecular dynamics in plant cells.

Source: Journal of Experimental Botany

DOI: 10.1093/jxb/eraf417

Authors: Sven zur Oven-Krockhaus, Leander Rohr, Luiselotte Rausch, Klaus Harter

Institution: University of Tübingen

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $4,341

Funders: Deutsche Forschungsgemeinschaft, Courant Forschungszentrum Geobiologie, Georg-August-Universität Göttingen