Spatiotemporal dynamics and selectivity of mRNA translation during mouse pre-implantation development
Year: 2025
Type: article
Abstract: Translational regulation plays a pivotal role during pre-implantation development. However, the mechanisms by which messenger RNAs (mRNAs) are selectively regulated over time, along with their dynamic utilization and fate during this period, remain largely unknown. Here, we performed fraction-resolved polysome profiling and characterized translational dynamics across oocytes and early embryo development. This approach allowed us to examine the changes in translation during pre-implantation development in high resolution and uncover previously unrecognized modes of translational selectivity. We observed a stage-specific delay in translation, characterized by the postponed recruitment of stored mRNAs-either unbound or associated with light ribosomal fractions-into actively translating polysomes (heavy fraction). Comparative analysis of translatome with proteomics, RNA N6-methyladenosine modifications, and mRNA features further revealed both coordinated and distinct regulatory mechanisms during pre-implantation development. Furthermore, we identified a eukaryotic initiation factor 1A domain containing 3, Eif1ad3, which is exclusively translated at the two-cell stage and is essential for embryonic development by regulating ribosome biogenesis and protein synthesis. Collectively, our study provides a valuable resource of spatiotemporal translational regulation in mammalian pre-implantation development and highlights a previously uncharacterized translation initiation factor critical for early embryos.
Source: Nucleic Acids Research
DOI: 10.1093/nar/gkaf956
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,992
Funders: U.S. Department of Agriculture, Ministry of Higher Education and Scientific Research, Grantová Agentura České Republiky, National Institutes of Health, National Institute of Food and Agriculture, National Institute of Child Health and Human Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development
Awards: G1025883367, G1190388083, G2075673663, G2180582178, From meiosis to mitosis - is starting a new life under in vitro conditions equivalent to that in vivo?, Signaling pathways regulating genome integrity during oocyte-to-embryo transition, G5266603089, G5846184781, Regulation of acentriolar spindle assembly and chromosome segregation in human and mouse oocyte meiosis, G6729332927, G8161559974, G9000139735
OpenAlex ID: W4414436263