Work

Analytical capability of Raman spectroscopy to detect biochemical changes in red blood cell membrane disorders

Year: 2025

Type: article

Abstract: Detection of RBC membrane disorders with currently available modalities, such as osmotic fragility test (OFT), EMA binding test, ektacytometry, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), and next-generation sequencing (NGS), has been challenging as they either lack biochemical inferences or are complex in nature. Raman spectroscopy, a highly analytical method known to produce molecular fingerprints, has proven potential in extracting biochemical information from single individual cells. Recent advancements in membrane-targeted Raman measurements using excitation spots of donut and line intensity profiles can transform lab-on-chip Raman-activated cell sorting methods into a potential technique for RBC membrane disorder diagnosis. We, therefore, conjecture that Raman spectroscopy can be a strong contender as a diagnostic modality in RBC membranopathies. In this comprehensive review, we have attempted to encompass the disorder-specific molecular defects, present diagnostic modalities, and their limitations, and explored the translational possibility of Raman spectroscopy as a diagnostic tool for membranopathies.

Source: Methods

DOI: 10.1016/j.ymeth.2025.09.007

Authors: Panchanil Sarmah, Ruchee Khanna, Aseefhali Bankapur

Institutions: Manipal Academy of Higher Education, Kasturba Medical College, Manipal

Language: English

Topic: Spectroscopy Techniques in Biomedical and Chemical Research

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,600