Work

Medial septum-dependent encoding of contextual inputs by hippocampal splitter cells

Year: 2025

Type: article

Abstract: The brain's ability to adapt to changing environments relies on encoding associations between stimuli and response strategies. Hippocampal splitter cells, which exhibit context-dependent firing patterns, are thought to contribute to this process, but whether they encode non-spatial contextual information and the neural circuits supporting such representations remains unclear. Using in vivo calcium imaging, we record dorsal hippocampal activity in mice performing a conditional discrimination task. Splitter cells encode both contextual input and response information in a dissociable manner, with their numbers and task-specific representations increasing during learning. Selective inhibition of the medial septum (MS) pathway or parvalbumin-expressing neurons in the MS reduces behavioral accuracy and decreases splitter cell numbers. MS inactivation specifically disrupts contextual representation without affecting response-related encoding. These findings demonstrate that splitter cells rely on distinct circuits to encode different components of the associative structure, with the MS critically supporting contextual inputs required for flexible behavior.

Source: Cell Reports

DOI: 10.1016/j.celrep.2025.116338