Year: 2025
Type: article
Abstract: Extracellular vesicles are cell-originated lipid bilayer membrane vesicles that play vital roles in cell-to-cell communications. While extracellular vesicles hold substantial biomedical potential, conventional methodologies for isolating extracellular vesicles require elaborate preprocessing and, therefore, remain labour intensive and limited by throughput. To overcome these challenges, we present a facile fabrication route for generating a meso-macroporous hydrogel matrix with pores of ~400 nm for customizable extracellular vesicle isolation. By combining surface charge-selective capture of extracellular vesicles within the hydrogel matrix and their recovery by high ionic strength, we report direct extracellular vesicle isolation with a throughput range from microlitre to litre scales, without preprocessing, for various biofluids, including whole blood, plasma, ascites, saliva, urine, bovine milk and cell culture media. Furthermore, we demonstrate that the meso-macroporous hydrogel also serves as a solid-phase matrix for preserving extracellular vesicles for on-demand downstream analyses, making it applicable for therapeutics, cosmeceuticals and disease diagnostics.
Source: Nature Nanotechnology
Authors: Junbeom Kim, Minjin Kang, Geonhee Han, Sujin Hyung, Mina Kim, Minjeong Jang, Han Kyul Lee, Yunhee Seo, Ki Cheol Gil, Changheon Kim, Sojin Song, Seonghyeok Jeong, Seongchan Kim, Min Soo Kim, Ji Sung Shim, Sung Gu Kang, Young Chan Lee, Seok Chung, Il-Joo Cho, Tae Soup Shim, Kwang Hoon Song, Jouha Min, Hyejeong Seong, Kyungeun Lee, Jeeyun Lee, Cheolju Lee, Hong Nam Kim, Hyojin Lee, Sun Hwa Kim, Ji Yoon Kang, Ki Wan Bong, Nakwon Choi
Institutions: Korea University, University of Michigan, Korea Institute of Science and Technology, Samsung Medical Center, Sungkyunkwan University, Korea University of Science and Technology, Korea Institute of Radiological and Medical Sciences, Kyung Hee University, Gyeongsang National University, Kyung Hee University Hospital at Gangdong, Ajou University, Incheon National University, U-M Rogel Cancer Center, Korea University Guro Hospital
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $12,690
Awards: G1274079493, G1370865451, G146827410, G1562100974, G1701622150, G1887594713, G197794407, G2406441932, G2574390382, G2974737859, G3502181266, G3694807721, G391878081, G4075562661, G4152714009, G4870493761, G4956025664, G4981271048, G5890639852, G5962814935, G6653693856, G6697169574, G6892706249, G7154269484, G7472590456, G8124493376, G856307194
OpenAlex ID: W4414478463