Work

Antibiotic susceptibility profile of clinical isolates of Clostridium species from a tertiary care hospital in North India: A prospective study

Year: 2025

Type: article

Abstract: : Purpose This study aims to identify the distribution of different clostridial species in human infections, sample types, and their antimicrobial susceptibility profile using the E-test method. Materials and methods The samples were processed as per the standard bacteriological techniques. Automated anaerobic gas evacuation-replacement system was used to create anaerobiosis (85% N 2 , 10% CO 2 , 5% H 2 ). Antibiotic susceptibility of isolated Clostridium species was performed using gradient diffusion (E strip) method against metronidazole, clindamycin, and penicillin. Results On antibiotic susceptibility testing by E-test, we have found 96.77% susceptibility to metronidazole, 90.32% susceptibility to penicillin, and 74.19% susceptibility to clindamycin. Conclusion It can be inferred that the E-test, when performed with the right inoculum, and when given the adequate period of incubation, could be used to reliably perform AST in Clostridium species. It produces a reliable report within 96 hours of sample processing.

Source: Indian Journal of Medical Microbiology

DOI: 10.1016/j.ijmmb.2025.100981

Authors: Manharpreet Kaur, Anjali Anil, Mani Bhushan Kumar, Cherring Tandup, Pallab Ray, Archana Angrup

Institutions: Christian Medical College, Post Graduate Institute of Medical Education and Research

Language: English

Topic: Bacterial Identification and Susceptibility Testing

Subfield: Clinical Biochemistry

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,210