Figure 3 from SALL4 Is Required for YAP1-Dependent Malignant and Regenerative Hepatocyte-to-Cholangiocyte Reprogramming
Year: 2025
Type: other
Abstract: Sall4 overexpression disrupts YAP1-driven HC-to-CCA transformation while enhancing the clonal expansion of fatty LPC-like cells. A, Schematic representation of the experimental design. FVB mice underwent HDTVI with transposon vectors expressing HA-tagged myrAkt and Yap1S127A (AY) combined with GFP expression (AY-GFP) or V5-tagged Sall4 (AY-Sall4) plasmids and were analyzed at indicated time points (2.5 and 5 weeks after HDTVI). B, qRT-PCR showing Sall4 expression in AY-Sall4 compared with AY-GFP. C, Representative liver images from AY-GFP or AY-Sall4 mice at 2.5 (top) and 5 weeks (bottom) after HDTVI. D, LW/BW ratio analysis comparing AY-GFP and AY-Sall4 at indicated time points. E and O, IHC analysis for indicated markers (HA-tag, panCK, HNF4A, Ki-67, and V5-tag) at indicated time points. F–N, Quantification of indicated parameters [tumor area, panCK+ area, lipid droplet area, Ki-67+ cells, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), bilirubin, CD45, TUNEL, and α-SMA] comparing AY-GFP and AY-Sall4. Data are presented as the mean ± SD; Student t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001). Scale bars, (E and O) 1 mm (overview), 200 μm (high magnification); (K) 200 μm. ns, not significant.
Language: English
Subfield: Hepatology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4414511307
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