Antibacterial and Antibiofilm Properties of Kulim Bark (Scorodocarpus borneensis Becc.) Hydrogel on Polymicrobial Biofilms
Year: 2025
Type: article
Abstract: Objectives: The study aimed to analyze the antibacterial and antibiofilm activity of hydrogels from Kulim (Scorodocarpus borneensis Becc.) stem bark extract and fraction against polymicrobial biofilms.Methods: The stem bark extract and fraction of Kulim (Scorodocarpus borneensis Becc.) were formulated into a hydrogel (tween 80:glycerin).Antibacterial and antibiofilm activities were assessed using a microdilution test against polymicrobial growth and polymicrobial biofilms, while acute toxicity was evaluated using wader pari (Rasbora lateristriata) embryos.Results: Kulim extract hydrogel (4 μg/μL) showed 69.16% antibacterial activity and 61.25% biofilm inhibition, with 72.58% and 82.86% biofilm degradation at 24 and 48 hours, respectively.The fraction-based hydrogel (0.22 μg/μL) had 58.10% antibacterial and biofilm inhibition activity, with 81.14% and 79.64% degradation at 24 and 48 hours.MIC 50 and MBIC 50 values were 2.42 μg/μL (extract) and 0.15 μg/μL (fraction) as well as 2.83 μg/μL and 0.19 μg/μL, respectively.Based on observation, MBEC 50 values were 1.09 μg/μL and 0.57 μg/μL (24 h), as well as 0.70 μg/μL and 0.03 μg/μL (48 h), respectively, while the extract's LC 50 was 0.143 mg/mL.Conclusion: The hydrogel made from Kulim stem bark extract exhibited stronger antibacterial and antibiofilm activities than the ethyl acetate fraction against polymicrobial.
Source: Journal of pharmacopuncture
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4414520259
FWCI:
Cites:
Cited by:
Related to: