Identification of Strobilanthes crispa from Its Related Plant Using Thin-layer Chromatography Fingerprint Analysis
Year: 2025
Type: article
Abstract: Strobilanthes crispa known as keji beling in Indonesia, belong to the Acanthaceae family. Thin layer chromatography (TLC) fingerprint pattern analysis is an excellent technique for evaluating and controlling the quality of raw materials containing Strobilanthes crispa. TLC fingerprints can be used to analyze the fingerprint profiles of medicinal plants for identification, authentication, and discrimination from related plants. This study aimed to develop a TLC fingerprint analysis method to identify S. crispa for quality control. Eleven bands were effectively separated using a silica gel 60 F254 TLC plate with a mobile phase of dichloromethane, ethyl acetate, and chloroform at 16:3.2:0.8. The derivatization reagent used to detect the separated bands was 10% sulfuric acid at a wavelength of 366 nm. Validation of the TLC fingerprint analysis, which evaluated the robustness, stability, specificity, and precision, met these requirements. S. crispa can be distinguished by its TLC fingerprint from sirih hutan (Piper aduncum), a related plant with similar leaf morphology. This approach could be used to identify and authenticate of S. crispa from P. aduncum.
Source: Majalah Obat Tradisional
DOI: 10.22146/mot.95008
Institutions: IPB University, Pakuan University
Language: English
Subfield: Complementary and alternative medicine
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4414520905
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